Fig. 4

TSPAN12 was associated with RNF152. a The interaction protein network of RNF152 revealed by the BioGRID database (https://thebiogrid.org/). b The cell lysates from 293 T cells transfected with empty vector (EV) or Flag-RNF152 were subjected to immunoprecipitation (IP) with Flag M2 beads, followed by immunoblotting with indicated antibodies. c The cell lysates of MG132-treated HuH6 cells were subjected to immunoprecipitation with IgG or anti-RNF152 antibody and detected by immunoblotting with indicated antibodies. d 293 T cells transfected Flag-tagged TSPAN12 plasmid were lysed and incubated with GST alone or GST-RNF152 immobilized on GST-Sepharose beads. The bound proteins were then detected by immunoblotting with indicated antibodies