Fig. 3

Suppression of ZEB2 inhibited proliferation, migration, and invasion while induced apoptosis of breast cancer cells. a–k MCF-7 and MDA-MB-231 cells were transfected with si-NC or si-ZEB2. a, b The expression of ZEB2 was detected by RT-qPCR assay in MCF-7 and MDA-MB-231 cells. c–e MTT and colony-forming assays were performed in MCF-7 and MDA-MB-231 cells. f, g The migration and invasion of MCF-7 and MDA-MB-231 cells were assessed by transwell assay. h The apoptosis was examined by flow cytometry assay. i–k The western blot assay was used to test protein expression of E-cadherin and Vimentin in MCF-7 and MDA-MB-231 cells. **P < 0.01, ***P < 0.001