Fig. 1

Flowchart of data collection and analysis in present study. Transcriptional profiles were collected from TCGA (PAAD)/ICGC (PACA-AU)/GEO (GSE28735 and GSE62452). Three immune clusters were derived by consensus clustering with five gene modules. The enrichment scores of cell types were calculated by X-cell. Then, comparison analysis in immune cell composition and immunomodulators expression were performed in groups and whole cohort. Combined with survival data of the patients TCGA/ICGC/GEO cohort, the prognostic analysis with immune features (gene modules, immune cells, and immunomodulators) were performed. To find promising targets for anti-PD-1/PD-L1 treatment, DEGs analysis and correlation analysis were performed in GEO (GSE28735 and GSE62452) database. The results led to gene TGM2, an oncogenic target for PDAC. The comparison analysis and prognostic analysis were performed in high-/low- TGM2 groups