Fig. 3

HOXA-AS3 knockdown suppresses NF-κB signaling in GC. a The impact of HOXA-AS3 knockdown on NF-κB luciferase reporter activity was analyzed in GC cells. b The effect of HOXA-AS3 knockdown on the expression of NF-κB-related signaling components in GC cell lines was evaluated via Western blotting, with GAPDH as a loading control. c, d The effect of HOXA-AS3 knockdown on the nuclear localization of p65 was assessed via immunofluorescent staining in MKN-45 (c) and SGC-7901 cells (d) (scale bar, 25 μm). Error bars: mean ± SD from triplicate experiments. ****P < 0.0001