Fig. 3
![Fig. 3](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs12935-021-01827-w/MediaObjects/12935_2021_1827_Fig3_HTML.png)
HOXA-AS3 knockdown suppresses NF-κB signaling in GC. a The impact of HOXA-AS3 knockdown on NF-κB luciferase reporter activity was analyzed in GC cells. b The effect of HOXA-AS3 knockdown on the expression of NF-κB-related signaling components in GC cell lines was evaluated via Western blotting, with GAPDH as a loading control. c, d The effect of HOXA-AS3 knockdown on the nuclear localization of p65 was assessed via immunofluorescent staining in MKN-45 (c) and SGC-7901 cells (d) (scale bar, 25 μm). Error bars: mean ± SD from triplicate experiments. ****P < 0.0001