Fig. 5

The HOXA-AS3/miR-29a-3p axis regulates LTβR expression in GC. a TargetScan was used to identify putative miR-29a-3p binding sites in the LTβR 3′-UTR, with mutated residues in reporter constructs being as indicated. b, c The impact of miR-29a-3p overexpression on WT and MUT LTβR luciferase reporter activity in MKN-45 (b) and SGC-7901 (c) cells was measured. d LTβR expression was negatively correlated with that of miR-29a-3p in 82 GC patient tumor samples. e, f Relative LTβR expression was evaluated via qPCR in MKN-45 (e) and SGC-7901 (f) cells after miR-29a-3p mimic or inhibitor transfection. g, h Western blotting was utilized to assess LTβR protein expression in MKN-45 (g) and SGC-7901 (h) cells following miR-29a-3p mimic or inhibitor transfection, with GAPDH as a loading control. i LTβR expression was positively correlated with that of HOXA-AS3 in 82 GC patient tumor samples, as assessed via qPCR. j Relative LTβR expression in MKN-45 (e) and SGC-7901 (f) cells following shHOXA-AS3-2 and/or miR-29a-3p inhibitor transfection. k, l LTβR protein levels were measured in GC cells transfected with shHOXA-AS3-2 and/or miR-29a-3p inhibitor via Western blotting, with GAPDH as a loading control. Error bars: mean ± SD from triplicate experiments. ^****P < 0.0001. NS no significance