Fig. 4

ATF3 alters stemness and EMT signature of SGC-7901 and MGC-803 cells in vitro. a, b Tumorsphere morphology imaging for ATF3 overexpression cells SGC-7901 and MGC-803. c, d EPCAM⁺/CD44⁺ subpopulation in ATF3 overexpression SGC-7901 and MGC-803 cells determined by flow cytometry. e, f qRT-PCR assay performed to assess the effect of ATF3 overexpression on the gene expression of EMT and stemness markers in SGC-7901 and MGC-803 cells. g, h Western blot of Twist1, Snai1, Slug, and CD44 in SGC-7901 and MGC-803 cells. These results are representative of at least three independent experiments (**p < 0.01; ***p < 0.001; NS: no significance)