Fig. 5

Down-regulation of SNHG6 suppressed the HCC growth in vivo. a The tumor growth ability of normal Huh7 cells and stable SNHG6 down-regulated Huh7 cells was evaluated using nude mice. b The tumor size was calculated. c The proliferated cells in the HCC tissues were labeled with Ki67 using immunohistochemistry methods. Ki67 positive cells were labeled brown. d Ki67 positive cells per field were calculated as the index of the proliferation status in the tumor tissues. e The levels of HIF1A, Sox2, Ki67 and cleaved caspase3 in the tumor tissues after SNHG6 knockdown. f Representative H&E staining images of the morphology of metastatic nodules in the lung of nude mice injected with Huh7 cells through the tail vein. g Quantification of the number of metastatic nodules in the lung. ***P < 0.001. Data were presented as mean ± SD from 6 independent experiments