Fig. 2

LncRNA VIM-AS1 promotes bladder cancer cell invasion and migration. a, f VIM-AS1 was knocked down in the high-metastatic bladder cancer cell line T24 by transfection of si1-VIM-AS1 or si2-VIM-AS1 and VIM-AS1 was overexpressed in the low-metastatic bladder cancer cell line RT24 by transfection of the VIM-AS1-overexpressing vector. si-NC or NC vector was used as a negative control. Transfection efficiency was confirmed by real-time PCR. si1-VIM-AS1 was selected for further experiments due to its better transfection efficiency. b, g The protein content and distribution of Vimentin in T24 and RT24 cells were detected using immunofluorescence (IF) staining (×400). c, h The protein levels of Snail, E-cadherin and Vimentin in T24 and RT24 cells were determined by immunoblotting. d, i The invasive ability of T24 and RT24 cells was determined by Transwell assay. Scale bar = 100 µm. e, j The migratory ability of T24 and RT24 cells was determined by wound healing assay. Scale bar = 100 µm. *P < 0.05, **P < 0.01