Fig. 2
From: Human drug efflux transporter ABCC5 confers acquired resistance to pemetrexed in breast cancer
Correlation between the IC50 values of MTA and expression of ABCC5 mRNA in primary cell lines from breast cancer (BC) patients (r = 0.741; p < 0.001, Pearson’s correlation test). The primary BC cells were cultured with a series of concentrations of MTA for 72 h, and then used to extract RNA for subsequent real-time RT-PCR. The dots represent means of three cell viability assays performed in triplicate, and 18S rRNA was used as an endogenous control