Fig. 4
From: Human drug efflux transporter ABCC5 confers acquired resistance to pemetrexed in breast cancer
Intracellular concentration–time curve of MTA influenced by the expression of ABCC5 in MCF-7 cells. Data represent mean ± SD (n = 3), *p < 0.05. The cells were incubated at 37 °C with 50 μM (21.37 μg/mL) MTA for 0, 0.5, 1, 2, 3, 4, and 24 h. After washing three times with cold phosphate buffered saline (0.1 M, pH 7.4), the cells were resuspended in RPMI-1640 (0.2 mL) and homogenized. MTA was quantified in the supernatant after centrifugation using RP-HPLC