Fig. 2

LINC01116 sponges miR-744-5p in prostate cancer cells. a, b FISH assay and nucleus-cytoplasm separation assay were utilized to determine the location of LINC01116. c RIP assay was implemented to assess the relationship of LINC01116 and Ago2. d The expression level of the five possible miRNAs was measured via qRT-PCR. e RNA pull down assay was conducted to detect the correlation of LINC01116 and miR-744-5p. f The alignment of LINC01116 and miR-744-5p were predicted by ENCORI. g The effectiveness of miR-744-5p mimics was assessed through qRT-PCR. h Luciferase reporter experiment was carried out for determining the binding relation of LINC01116 and miR-744-5p. **P < 0.01, n.s.: no significance