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Fig. 3 | Cancer Cell International

Fig. 3

From: CDC42EP3 promotes colorectal cancer through regulating cell proliferation, cell apoptosis and cell migration

Fig. 3

The effects of CDC42EP3 knockdown on cell proliferation, colony formation and cell apoptosis. a MTT was performed to estimate the effects of CDC42EP3 knockdown on cell proliferation. b EdU staining was performed to show the proliferative activity of RKO and HCT116 cells with or without CDC42EP3 knockdown. c Number of colonies formed by RKO and HCT116 cells with or without CDC42EP3 knockdown was counted. d Flow cytometry was employed to evaluate the cell apoptosis of RKO and HCT116 cells with or without CDC42EP3 knockdown. e Human Apoptosis Antibody Array was utilized to identify differentially expressed apoptosis-related proteins, by which CDC42EP3 regulated cell apoptosis. The representative images were randomly selected from at least 3 independent experiments in duplicate. *P < 0.05, **P < 0.01, ***P < 0.001

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Cancer Cell International

ISSN: 1475-2867

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