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Table. 3. Cell viability assays employed in the evaluation of drug efficacy using MCTs

From: Challenges of applying multicellular tumor spheroids in preclinical phase

Assay type Culture model Cancer cell Drugs Readout References
CellEvent Caspase-3/7 Green 2D Prostate cancer (VCaP, LNCaP) MLN4924 Spheroid [107]
LysoTracker Deep Red 2D Prostate cancer (VCaP, LNCaP) MLN4924 Spheroid [109]
Annexin V-APC 2D Breast cancer (MDA-MB-435S, MDAMB-468, MDA-MB-231, SK-BR-3) Disassociation [22]
MTT 2D Breast cancer (MCF-7) Tamoxifen Disassociation [97]
LDH 2D Breast cancer (MCF-7) Tamoxifen Disassociation [97]
AlamarBlue 2D Lung cancer (H460, A549, H1650) Cisplatin, Gemcitabine
5-fluorouracil, Camptothecin
Disassociation [59]
2D Breast cancer (MCF-7) Doxorubicin Spheroid [69]
Live/Dead 2D Breast cancer (MCF-7) Doxorubicin Spheroid [110]
2D Breast cancer (MCF-7) Spheroid [73]
Cultrex® 3D Colorimetric 3D Lung cancer (A549) 4-HPR-HSA Spheroid [106]
CellTiter-Glo Luminescent 3D Glioblastoma (U-87 MG, KNS42)
Oral squamous (LICR-LON-HN4)
Breast carcinoma (MDA-MB-231)
HSP90 chaperone inhibitor
PI3 kinase/mTOR inhibitor
PLCg inhibitor
Spheroid [41]