Slug suppresses EpCAM expression in cervical cancer cells.
a Heatmap of the enriched differentially expressed genes identified by RNA transcriptome sequencing assays between the SiHa-Slug and SiHa-Vec groups: Slug, EpCAM, CDH1, MMP2, ZEB1, VIM, MMP9, ZEB2, TWIST1 and TWIST2; data were log10 normalized. b The mRNA level of EpCAM in Slug-modified cells was detected by real-time quantitative PCR, and the quantitative analysis is shown. The protein levels of EpCAM and E-cadherin were detected by western blotting: c SiHa-Vec and SiHa-Slug cells, d HeLa-Vec and HeLa-Slug cells, and e CaSki-shControl and CaSki-shSlug cells. The expression of Slug and EpCAM was detected in SiHa-Vec and SiHa-Slug cells by using immunofluorescence (f) and immunocytochemistry analyses (g), scale bars, 1000× and 400×. The expression of Slug and EpCAM was detected in mouse xenografted tumor tissues derived from SiHa-Vec and SiHa-Slug cells by using western blotting (h) and immunocytochemistry analysis (i), scale bars, 1000 ×. Data were statistically analyzed with Student’s t-test, and data are shown as the mean ± SD of three independent experiments. * p < 0.05 vs. control