Fig. 1

Upregulation of FZD2 in BC tissues is correlated with poor prognosis. a Data obtained from TCGA database revealed the FZD2 expression in different types of cancer tissues. b DMFS of BC patients with high or low level of FZD2 were analyzed by generating KM plots based on publicly available microarray data. c Representative images of FZD2 upregulation in BC tissues were generated from the ONCOMINE database. d qRT-PCR detected the mRNA level of FZD2 in 42 BC tissues and adjacent normal tissues. e Western blot analysis of FZD2 protein in BC tissues with adjacent normal tissues as controls. f Representative images of FZD2 expression in BC tissues were obtained using IHC (Original magnification, × 400, scale bar, 100 μm). g OS (left panel) and DMFS (right panel) in BC patients were analyzed by KM method. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 was a symbol of statistical significance. TCGA, the cancer genome atlas; FZD2, frizzled class receptor 2; BC, breast cancer; KM, Kaplan–Meier; OS, overall survival; RFS, recurrence-free survival; DMFS, distant metastasis free survival