Fig. 3

FZD2 promotes BC cell growth in vivo. a Images of tumors removed from two groups of mice. b Volumes of tumors derived from FZD2-downregulated cells or control cells. c Weights of tumors derived from FZD2-downregulated cells or control cells. d The positivity of TGF-β1 and Ki-67 in the tumor tissues collected from two groups of mice was detected by IHC (Original magnification, × 400, scale bar = 100 μm). Apoptosis in the tissues collected from two groups of mice was measured by TUNEL assay. e Apoptosis-related proteins were detected in two groups of tumor tissues. f qRT-PCR analysis of mRNA levels of E-cadherin, N-cadherin, Vimentin and TGF-β1 in tissues obtained from two groups of mice. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 was a symbol of statistical significance. FZD2, frizzled class receptor 2; BC, breast cancer