Skip to main content
Fig. 2 | Cancer Cell International

Fig. 2

From: Circ_0000745 strengthens the expression of CCND1 by functioning as miR-488 sponge and interacting with HuR binding protein to facilitate the development of oral squamous cell carcinoma

Fig. 2

Circ_0000745 downregulation inhibited OSCC development in vitro and in vivo. a The efficiency of siRNA sequences targeting circ_0000745 was examined by qPCR. In Cal-27 and SCC9 cells transfected with sh-circ_0000745#1 or sh-NC, (b) cell proliferation was assessed by CCK-8 assay. c Cell proliferation was also assessed by colony formation assay. d Cell apoptosis was determined by flow cytometry assay. e, f Cell cycle distribution was distinguished by flow cytometry assay. g The protein levels of PCNA, Cleaved-caspase 3 and CCND1 were detected by western blot. h, i The role of circ_0000745 in vivo was identified by animal studies, and tumor volume and tumor weight were measured to assess tumor growth. *P < 0.05, **P < 0.01 and ***P < 0.001

Back to article page

Cancer Cell International

ISSN: 1475-2867

Contact us