Fig.6

TGF-β1 was directly targets of miR-503-3p. a Putative targeting sites for TGF-β1 and miR-503-3p. b HepG2 cells and Huh7 cells were cotransfected with the miR-503-3p mimic and reporter plasmid containing the wild-type (WT) or Mut (MT) 3′-UTR of TGF-β1. A luciferase activity assay was subsequently performed. c HepG2 cells and Huh7 cells were cotransfected with the miR-503-3p inhibitor and reporter plasmid containing the wild-type (WT) or Mut (MT) 3′-UTR of TGF-β1. A luciferase activity assay was subsequently performed. d Protein expression levels of TGF-β1 after transfection with miR-503-3p mimic and inhibitor in HepG2 cells and Huh7 cells. e Expression levels of miR-503-3p was increased after exposure of ECH in HepG2 and Huh7 cells. N = 6, means ± SD, VS the 0 group, * p < 0. 05, **p < 0.01