Fig. 6

miR-29a inhibited the proliferation and invasion, but induced apoptosis of osteosarcoma cells via targeting NFIA. a–b NFIA was knocked down in both mRNA and protein levels. c–d The cck-8 assay. e Colony formation assay. f The EdU assay for proliferation. g Flow cytometry for apoptosis. h The transwell assay for migration and invasion. i The wound-healing assay. j The expression levels of NFIA, apoptosis-related, and EMT-related proteins were detected by western blot. Inhibitor + siNFIA: co-transfecting miR-29a inhibitor and siNFIA. *p < 0.05, **p < 0.01, compared with control group; &p < 0.05, &&p < 0.01, compared with miR-29a inhibitor group. ns = no significant