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Fig. 6 | Cancer Cell International

Fig. 6

From: Morphological and molecular characteristics of spheroid formation in HT-29 and Caco-2 colorectal cancer cell lines

Fig. 6

Flow cytometry analysis of CRC-CSC markers expression in HT-29 and Caco-2 spheroids compared to their parental cells. Representative flow cytometry plots confirmed both HT-29 and Caco-2 spheroids show higher expression of CD166, CD44 and CD133 CRC-CSC markers as compared to their parental cells. Upregulation of CSC markers in spheroids as compared to their differentiated counterparts was predominant in Caco-2 spheroids than HT-29 spheroids. Dot plots show the expression of each marker in one representative experiment

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