Table1 Primers used for quantitative RT-PCR
Genes groups | Gene name | Primer Sequence (5´ → 3´) |
|---|---|---|
Housekeeping gene | GAPDH | F-CATGAGAAGTATGACAACAGCCT R-AGTCCTTCCACGATACCAAAGT |
Stemness genes | C-MYC | F-ACACATCAGCACAACTACG R-CGCCTCTTGACATTCTCC |
KLF4 | F-CCTCGCCTTACACATGAAGAG R-CATCGGGAAGACAGTGTGAAA | |
SOX2 | F-AATGGGAGGGGTGCAAAAGAGG R-GTGAGTGTGGATGGGATTGGTG | |
NANOG | F-AGCTACAAACAGGTGAAGAC R-GGTGGTAGGAAGAGTAAAGG | |
OCT4-A | F-GTGGAGAGCAACTCCGATG R-TGCAGAGCTTTGATGTCCTG | |
EMT genes | Vimentin | F-TCTACGAGGAGGAGATGCGG R-GGTCAAGACGTGCCAGAGAC |
SNAIL1 | F-CCAGAGTTTACCTTCCAGCA R-GATGAGCATTGGCAGCGA | |
TWIST1 | F-TTCTCGGTCTGGAGGATGGA R-CCACGCCCTGTTTCTTTGAAT | |
N-cadherin | F-GCCCAAGACAAAGAGACCC R-CTGCTGACTCCTTCACTGAC | |
E-cadherin | F-CAGGAGTCATCAGTGTGGT R-GGAGGATTATCGTTGGTGTCAG | |
ZEB1 | F-CTTCTCACACTCTGGGTCTTATTC R-CGTTCTTCCGCTTCTCTCTTAC | |
ABC Transporte | ABCG2 | F-TTCCACGATATGGATTTACGG R-GTTTCCTGTTGCATTGAGTCC |
ABCB1 | F-GTTCAGGTGGCTCTGGATAAG R-AGCGATGACGTCAGCATTAC | |
ABCC1 | F-CGCCTTCGCTGAGTTCCT R-TGCGGTGCTGTTGTGGTG |