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Table1 Primers used for quantitative RT-PCR

From: Morphological and molecular characteristics of spheroid formation in HT-29 and Caco-2 colorectal cancer cell lines

Genes groups Gene name Primer Sequence (5´ → 3´)
Housekeeping gene GAPDH F-CATGAGAAGTATGACAACAGCCT
R-AGTCCTTCCACGATACCAAAGT
Stemness genes C-MYC F-ACACATCAGCACAACTACG
R-CGCCTCTTGACATTCTCC
KLF4 F-CCTCGCCTTACACATGAAGAG
R-CATCGGGAAGACAGTGTGAAA
SOX2 F-AATGGGAGGGGTGCAAAAGAGG
R-GTGAGTGTGGATGGGATTGGTG
NANOG F-AGCTACAAACAGGTGAAGAC
R-GGTGGTAGGAAGAGTAAAGG
OCT4-A F-GTGGAGAGCAACTCCGATG
R-TGCAGAGCTTTGATGTCCTG
EMT genes Vimentin F-TCTACGAGGAGGAGATGCGG
R-GGTCAAGACGTGCCAGAGAC
SNAIL1 F-CCAGAGTTTACCTTCCAGCA
R-GATGAGCATTGGCAGCGA
TWIST1 F-TTCTCGGTCTGGAGGATGGA
R-CCACGCCCTGTTTCTTTGAAT
N-cadherin F-GCCCAAGACAAAGAGACCC
R-CTGCTGACTCCTTCACTGAC
E-cadherin F-CAGGAGTCATCAGTGTGGT
R-GGAGGATTATCGTTGGTGTCAG
ZEB1 F-CTTCTCACACTCTGGGTCTTATTC
R-CGTTCTTCCGCTTCTCTCTTAC
ABC Transporte ABCG2 F-TTCCACGATATGGATTTACGG
R-GTTTCCTGTTGCATTGAGTCC
ABCB1 F-GTTCAGGTGGCTCTGGATAAG
R-AGCGATGACGTCAGCATTAC
ABCC1 F-CGCCTTCGCTGAGTTCCT
R-TGCGGTGCTGTTGTGGTG