Fig. 5

Cereblon was a powerful helper for ARV-825 in T-ALL cells. a Western blot analysis of CRBN protein expression in T-ALL cells. b Overexpressing CRBN in 6 T-CEM, Molt4 and Jurkat cells with pLX304-CRBN-V5 lentivirus at 5 days. OE: overexpression. c Knockdown of CRBN expression by sh-CRBN lentivirus at 5 days in 6 T-CEM, Molt4 and Jurkat cells. Scr: Scramble; KD: knockdown. d Comparison of sensitivity to ARV-825 between cells overexpressing CRBN and cells transfected by empty vector alone in 6 T-CEM, Molt4 and Jurkat cells. The results showed excess CRBN reduced the IC50 of ARV-825 in these cells. OE: overexpression. e Sensitivity to ARV-825 of cells transfected with sh-CRBN and sh-scramble in 6 T-CEM, Molt4 and Jurkat cells showed that downregulated CRBN increased the IC50 of ARV-825 in these cells