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Fig. 2 | Cancer Cell International

Fig. 2

From: MicroRNA-665 facilitates cell proliferation and represses apoptosis through modulating Wnt5a/β-Catenin and Caspase-3 signaling pathways by targeting TRIM8 in LUSC

Fig. 2

miR-665 enhances LUSC NCI-H226/SK-MES-1 cell growth and reduces apoptosis. a miR-665 expressions increased in NCI-H226/SK-MES-1 cells after transfection with the miR-665 mimics. b miR-665 expressions decreased after transfection with the miR-665 inhibitor. c CCK8 results showed that miR-665 mimics facilitated LUSC cell proliferation at 48, 72 and 96 h after transfection. d CCK8 results showed that miR-665 inhibitor repressed LUSC cell proliferation at 48, 72 and 96 h after transfection. e Flow cytometry analysis of cell cycle. The proportion of cells changed in the G0/G1, S and G2/M stages after transfection with miR-665 mimics. f The ratio of cells changed in the G0/G1, S and G2/M stages after transfection with miR-665 inhibitor. g The percentage of sub-G1 cells population changed after transfection with miR-665 inhibitor. h Flow cytometry analysis of cell apoptosis. The ratios of early and late apoptosis reduced after transfection with miR-665 mimics. i The ratios of early and late apoptosis increased after transfection with miR-665 inhibitor. j PCNA protein expression upregulated after transfection with the miR-665 mimics. k PCNA protein expression downregulated after transfection with the miR-665 inhibitor. n = 3, *p < 0.01, **p < 0.001.

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Cancer Cell International

ISSN: 1475-2867

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