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Fig. 5 | Cancer Cell International

Fig. 5

From: MicroRNA-665 facilitates cell proliferation and represses apoptosis through modulating Wnt5a/β-Catenin and Caspase-3 signaling pathways by targeting TRIM8 in LUSC

Fig. 5

TRIM8 siRNA facilitates human LUSC cell proliferation and inhibits apoptosis. a qRT-PCR showed the silencing efficiency of TRIM8 siRNA in NCI-H226/SK-MES-1 cells at 24 h after transfection. b CCK8 assay revealed that TRIM8 siRNA promoted the proliferation of NCI-H226/SK-MES-1 cells. c Flow cytometric analysis showed that G0/G1 phase cells reduced after TRIM8 siRNA transfection, while S and G2/M phase cells were increased. d Apoptosis assay revealed the proportion of early and late apoptosis after TRIM8 siRNA transfection. e Wnt5a, β-Catenin, c-Myc, Cyclin D1 and active Caspase-3 protein expressions were detected at 48 h after TRIM8 siRNA transfection. f TRIM8 siRNA inhibited Wnt5a degradation in NCI-H226/SK-MES-1 cells. 10 μg/mL cycloheximide (CHX) was used to suppress de novo Wnt5a biosynthesis. g TRIM8 siRNA affected TOPFLASH TCF-reporter construct activity in NCI-H226/SK-MES-1 cells. h CCK8 assay revealed cell proliferation after co-transfection with TRIM8 siRNA and Wnt5a siRNA in NCI-H226/SK-MES-1 cells. n = 3, *p < 0.01, **p < 0.001, as compared with NC-siRNA group; #p < 0.01, as compared with TRIM8 siRNA group

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Cancer Cell International

ISSN: 1475-2867

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