Fig. 2

Knockdown of BET expression or inhibition of BET activity suppressed the growth of osimertinib resistant cells. a, b osimertinib resistant (H1975-OR) cells were transfected with siRNAs pools that specifically target BRD2, 3, or 4 for 24 h, followed by another 24 h incubation before being subjected to western blot analysis (a) or reseeded to 96-well plates for a 5-day SRB assay (b). c, d osimertinib sensitive parental (H1975-P) cells and H1975-OR cells were either treated with JQ1 for 3 days and subjected to sulforhodamine B (SRB) assays (c) or treated with JQ1 (1, 5, 10 μmol/L) for 24 h and subjected to western blot analysis (d). Points, mean; bars, SD; *, P < 0.05