Fig. 2

a–c Mouse tumour intensity outputs from MouseTensity of data captured on the XVI (a), the BIX (b) or the LPT (c) on day 24. Mouse 1, 2 and 3 are shown, grouped by cage in the XVI and the BIX. Mouse 1, 2 and 3 are shown separately as the images are acquired per mouse in the LPT. The highest intensity in each image is normalised to 1. (S.U.: Standardised Unit) A colour scale indicating highest and lowest values in indicated on the top right of A. d–f Representative example of mouse 2 on day 24. Data from XVI (d), BIX (e) or LPT (f) are visualised (S.U.: Standardised Unit). The section coloured rainbow is used as the measurement signal, while the monochrome purple is considered noise. The difference is delineated by a yellow line. g–i Measurements of fluorescent intensity of tumours over time as measured by the XVI (g) the BIX (h) or the LPT (i). Error bars represent ± SD, (ANOVA: asterisk indicates Tukey’s multiple comparisons test p < 0.05, NS p > 0.05). j Fold increase from average tumour size on day 17 to average tumour size on day 24. Measurements were calculated with each platform’s software. Error bars represent + SD. k Coefficients of variance for the software provided with each platform. Coefficient of variance was measured as a function of the final day. Error bars represent + SD