Fig. 3

Alkaline phosphatase (ALPL) inhibited the metastasis of LUAD cells in vivo and in vitro. Transwell assays were performed to determine the effect of ALPL overexpression on A549 (a) and HCC827 (c) cells, Scale bars: 100 µm. b, d The migration and invasion rate of A549 (a) and HCC827 (c) cells, n = 3. e A tail vein injection model was used to evaluate lung metastasis. The front and back images of the lungs are shown, n = 9. f Representative H&E images of A549 (Vector) and A549 (ALPL) tumors, Scale bars: 250 µm, 100 µm or 25 µm. g Quantification of the number of metastatic lung tumors in five pairs of nude mice. h Tube formation assay were performed to determine the effect of ALPL overexpression on the angiogenesis activity of A549 and HCC827, Scale bars: 100 µm. i Capillary length was calculated in A549 and HCC827 cell culture medium. Data were presented as the mean ± SD and analyzed by Student′s t-test. Asterisks (*) represent statistical significance (P < 0.05)