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Fig. 7 | Cancer Cell International

Fig. 7

From: GNG5 is a novel oncogene associated with cell migration, proliferation, and poor prognosis in glioma

Fig. 7

Knockdown of GNG5 expression in glioma cells can affect the functional phenotype of glioma cells. a The expression levels of GNG5 in different glioma cell lines and human astrocytes (HA) were analyzed based on RT-qPCR; b Efficiency comparison of different siRNA sequences for targeted knockdown of GNG5 in U251 cell line; c MTT assay results of U251 cell lines transfected with shGNG5 and shNC; d Clonogenic results of U251 cell lines transfected with shGNG5 and shNC; e analysis of clonogenic results; f Ki67 immunofluorescence staining results of U251 cell lines transfected with shGNG5 and shNC; g Analysis of Ki67 staining results; h Results of scratch healing ability assay of U251 cell lines transfected with shGNG5 and shNC; i Analysis of scratch healing capacity test results. ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05

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