Fig. 1

The expression of miR-190 in Human BC tissues and its role in bladder urothelial cell transformation. a The expression of miR-190 in BC tissues was compared with normal bladder tissues and miR-190 was up-regulated in BC tissues (p = 0.0118). b miR-190 and control vector were stably transfected into UROtsa. The symbol (*) indicates a significant increase (*P < 0.05, **P < 0.01, ***P < 0.001). c, d 1 × 10⁴ cells of UROtsa(pLKO.1) and UROtsa(miR-190) were subjected to soft agar assay in the presence of EGF (20 ng/ml). The images were captured under inverted microscopies after being incubated in a 37 °C with 5% CO₂ incubator for 3 weeks (c) and the colonies were also counted (d). Each bar indicates the mean ± SD from triplicate assays. The symbol (*) indicates a significant increase as compared with the medium control, while the symbol (#) indicates a significant increase in comparison to UROtsa(pLKO.1) cells (*P < 0.05, **P < 0.01, ***P < 0.001; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001). e miR-190 sponge inhibitor (miR-190 inhibitor) and control vector were stably transfected into UMUC3 cells, and the symbol (*) indicates a significant decrease (*P < 0.05, **P < 0.01, ***P < 0.001). f, g the indicated cell transfectants were subjected to soft agar assay same as described in “c, d” (f, g); The symbol (*) indicates a significant decrease as compared with the UMUC3(LacZ) cells (*P < 0.05, **P < 0.01, ***P < 0.001)