Fig. 6

Effect of aloe-emodin on apoptosis of HCT116 colon cancer cells. a DAPI staining (× 20 magnification), 40 μM 80 μM for 24 h (× 40 magnification) and the white arrow points to the saient features of apoptotic cells. b The cell cycle is assessed by flow cytometry. ***P < 0.001 versus Control. ^###P < 0.001 versus 20 μM. c Capase-3 activity assay. Data are expressed as mean ± SD. * P < 0.05, ***P < 0.001 versus Control. ^#P < 0.05 versus 20 μM. All the experimental data were collected from at least three independent experiments. Multiple comparisons using LSD method (b), Tamhane' T2 and one-way ANOVA (c)