Fig. 1

a Half maximal inhibitory concentration (IC50) demonstrates the increased resistance to carboplatin from the sensitive (HMPOS-S) to the most resistant cells (HMPOS-2.5R and HMPOS-10R), the IC 50 of HMPOS-10R is plotted in b (n = 3). b HMPOS 10 R IC50 exhibit a substantially high value in comparison to the other cell lines (n = 3). c Cell viability assay of generation 0 demonstrates a significant resistance to carboplatin at all concentrations for the HMPOS-10R cell lines. The HMPOS-2.5R cells exhibit resistance only at the highest concentration of the drug (n = 3, [*] = P < 0.05, [**] = P < 0.01, [***] = P < 0.001). d Sensitive and resistant HMPOS, Generation 10 cell lines. e Doubling time of HMPOS generation 0, sensitive and resistant cell lines (n = 3, [**] = P < 0.01, [***] = P < 0.001). A significant increase in doubling time was evident in HMPOS-2.5R (P < 0.01, [**]) and HMPOS-10R (P < 0.001, [***]) (n = 3) f Doubling time for HMPOS-S and resistant cell lines of generation 10 demonstrate that HMPOS-2.5 and 10R returned to the original doubling time (n = 3). g Doubling time between three generations (0, 5 and 10) of HMPOS-S show no statistically significant difference (n = 3). h Comparison of doubling time of HMPOS-2.5R through the examined generations (0,5,10) is significantly decreased with the later generations (n = 3, [***] = P < 0.001).i Cross generational comparison for the doubling time of HMPOS-10R also demonstrate a significant decrease in generations 5 and 10 (n = 3, [***] = P < 0.001)