Fig. 3
From: Knockdown of MCM8 inhibits development and progression of bladder cancer in vitro and in vivo
Loss-of-function of MCM8 ameliorated cell cycle and apoptosis of bladder cancer cells. a The effects of MCM8 knockdown on cell cycle were determined by flow cytometry. b The effects of MCM8 knockdown on cell apoptosis were examined by flow cytometry. c The expression of apoptosis-related proteins in T24 cells transfected with shMCM8 was measured by ECL with Human Apoptosis Antibody Array. The results circled in red indicated that the protein expression was up-regulated and P < 0.05. d Protein expression was presented in grayscale and visualized by R studio. e The expression levels of apoptosis-related proteins were analyzed in T24 cells with shMCM8. f The expression of Akt, P-Akt, CCND1, CDK6 and MAPK9 was detected by western blot. Results were presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001