Fig. 2

ILF3-AS1 interacts with miR-4306 in PTC cells. a, b The distribution of ILF3-AS1 in IHH-4 and TPC-1 cells was detected by subcellular fractionation assay and FISH assay. c Bioinformatics tool screened out 6 miRNAs that may have the ability to interact with ILF3-AS1. d RNA pull down assay was performed to test the interaction between 6 candidate miRNAs and ILF3-AS1. e The expression level of miR-4306 in PTC cell lines was assessed by RT-qPCR. f RNA pull down assay showed that ILF3-AS1 was pulled down abundantly by biotinylated miR-4306-WT. g The putative binding sites of miR-4306 with ILF3-AS1 were predicted. h The overexpressing efficacy of miR-4306 mimics was examined in IHH-4 and TPC-1 cells. i Dual Luciferase reporter assay was applied to prove the mutual interaction between ILF3-AS1 and miR-4306. ^*P < 0.05, ^**P < 0.01