Fig. 4

Decreased circRNA_100290 expression suppresses GC cell migration and invasion abilities and impedes EMT. A A wound healing assay was performed to evaluate the effects of circRNA_100290 on the migration abilities of AGS and HGC27 cells at 0 h, 24 h, and 48 h. B, C The statistical analysis of the graphs showed that knocking down circRNA_100290 obviously inhibited AGS and HGC27 cell wound healing (*P < 0.05). D, E Transwell migration assays were performed to evaluate the effects of circRNA_100290 on the migration abilities of AGS and HGC27 cells. F, G The statistical analysis of the graphs showed that decreased circRNA_100290 expression induced fewer AGS and HGC27 cells to migrate into the lower chamber compared with the control groups (*P < 0.05, ** P < 0.01). H, I Transwell invasion assays were performed to evaluate the effects of circRNA_100290 on the invasion abilities of AGS and HGC27 cells. J, K The statistical analysis of the graphs showed that reduced circRNA_100290 expression led fewer AGS and HGC27 cells invading the lower chamber compared with the control groups (*P < 0.05, ** P < 0.01). L Western blot assays were performed to evaluate the expression of EMT-related proteins, including E-cadherin, N-cadherin and Vimentin