Fig. 3
From: A novel phosphoramide compound, DCZ0805, shows potent anti-myeloma activity via the NF-κB pathway
DCZ0805 induces MM cells apoptosis. a Cells were treated with DCZ0805 for 48 h and analyzed by FACS using Annexin V/PI staining. Columns show the percentage of Annexin-V positive cells from three independent experiments, data shown as the means ± standard deviation. (*P < 0.05, **P < 0.01, ***P < 0.001 compared with the 0 µM group, or as indicated). b Cells were incubated with or without the pan-caspase inhibitor Z-VAD-FMK, and then treated with 15 µM DCZ0805 for 48 h, stained with Annexin V/PI and analyzed by FACS. Data are represented as mean ± SD of three independent experiments and **P < 0.01, by unpaired two-tailed Student's t tests, compared with the DCZ0805-treated group. c Detect the expression levels of apoptosis-related proteins by Western blot