Fig. 5
Dual inhibition of PI3K-AKT and autophagy pathways by 3-MA sensitizes MCL cells to BTZ in vitro. Jeko and REC-1 cells (a) as well as manipulated Jeko cells (b) were treated with BTZ (20 nM) in the presence or absence of 3-MA (5 mM) for 24 h. Cells treated with DMSO, 3-MA or BTZ plus CQ (20 μM) were used as negative controls. Cell viability was measured using MTT assays. Data are normalized to DMSO-treated cells and shown as the mean ± SD from three independent experiments. N.S, not significant; **p < 0.01; ***p < 0.001 (vs BTZ-treated group). c The synergic cytotoxic effects of BTZ and 3-MA in REC-1 and BACH2KD Jeko cells were further determined using the combination index (CI) based on the data from cell viability assays. Fa, fraction affected; B, BTZ; 3, 3-MA