Fig. 7

LncRNA WDFY3-AS2 induced the traits of CSCs by regulating miR-139-5p/SDC4. A Representative A2780-DDP sphere-forming cell images were acquired (100 μm). The transfected A2780-DDP cells were treated for 7 days with cisplatin (20 μM). B Western blotting was performed to assess CSC markers protein expression including the SOX2, OCT4 and Nanog in A2780-DDP sphere-forming cells. C CD44⁺, CD166⁺-positive cells in A2780-DDP sphere-forming cells detected via flow cytometry. D The weight of transplanted tumors in nude mice injected with si-WDFY3-AS2, miR-139-5p inhibitor or SDC4. E Assessment of tumors in nude mice. F The volume curve of xenograft tumors. ***P < 0.001 vs. control, ^###P < 0.001 vs. si-WDFY3-AS2 + inhibitor NC, ^&&&P < 0.001 vs. si-WDFY3-AS2 + pcDNA-NC