Fig. 6
From: FOXH1 promotes lung cancer progression by activating the Wnt/β-catenin signaling pathway
Knockdown of FOXH1 suppresses β-Catenin signaling in lung cancer. a Western blotting showing that FOXH1 knockdown decreased the expression of β-catenin, p-GSK-3β and cyclin D1 in A549 and PC9 cells. β-actin served as the internal control. Densitometry analyses were performed using ImageJ software and normalized to loading WB controls (***p< 0.001 vs. sh-NC). b TCF/LEF-dependent transcriptional activity of β-catenin in A549 and PC9 cells transfected with TOP/FOP flash reporter plasmids. Assays of relative luciferase activity in cells were performed (*p < 0.05 vs. sh-NC)