Fig. 4

MiR-493-3p is highly expressed in glioma tissues and correlated with progression and poor prognosis. A Schematic illustration exhibiting the overlap among the target miRNAs for circNALCN predicted by circBank, starBase, and sequencing up-regulated miRNA data. B Volcano plot of differentially expressed miRNAs. The blue dots represent downregulated miRNAs, whereas the red dots represent upregulated miRNAs in glioma compared with normal tissue. MiRNA is labeled as miR-493-3p. C miR-493-3p expression was detected via qRT-PCR in various glioma cell lines (U87, U251 and SF126) and human normal glial cell (HEB). D, E Relative expression levels of miR-493-3p in U87 cells (D) and U251 cells (E). F qRT-PCR was used to detect the relative expression of miR-493-3p in glioma tissues (n = 76) and normal brain tissues (n = 17). G Pearson’s correlation analysis between circNALCN and miR-493-3p expression based on glioma tissues. H Kaplan Meier survival analysis showed that the OS of glioma patients with high miR-493-3p expression was lower than those with low miR-493-3p expression. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001