Fig. 6

MiR-493-3p targeting PTEN promotes proliferation and migration of glioma cells in vitro. A qRT-PCR analyzed the expression of PTEN in U87 and U251 cells overexpressing PTEN. B, C Relative mRNA and protein levels of PTEN were evaluated by qRT-PCR (B) and western blot (C), respectively, in cells transfected with the miR-493-3p mimics and inhibitor. D Schematic illustration of PTEN-WT and PTEN-MUT luciferase reporter vectors. E, F After co-transfection with PTEN-WT or PTEN-MUT and miR-493-3p mimics or NC, the relative luciferase activity of U87 and U251 cells was detected. G RNA pulldown assay was executed in U87 and U251 cells, followed by qRT-PCR to detect the expression of miR-493-3p. H, I qRT-PCR (H) and western blot (I) were used to detect relative PTEN mRNA and protein levels, respectively, in U87 and U251 cells transfected with vector and miR-493-3p NC, vector and miR-493-3p mimics and miR-493-3p mimics and oe-PTEN. J, K CCK-8 was used to detect the proliferation abilities of U87 and U251 cells transfected with vector and miR-493-3p NC, vector and miR-493-3p mimics and miR-493-3p mimics and oe-PTEN. G, H The migration abilities of U87 and U251 cells transfected with vector and miR-493-3p NC, vector and miR-493-3p mimics, and miR-493-3p mimics and oe-PTEN were tested by wound healing test and Transwell assay. Scale bar: 100 µm. Data are expressed as the mean ± SD; ns indicates no significance. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Vector: empty vector control; NC: negative control oe-PTEN: PTEN overexpression vector; si-NC: negative control siRNA