Fig. 7

CircNALCN, as an miRNA sponge for miR493-3p, regulates the expression of PTEN. A qRT-PCR was used to detect the relative expression of PTEN in glioma tissues (n = 76) and normal brain tissues (n = 17). B Kaplan–Meier survival analysis showed that OS in glioma patients with the high expression of PTEN was higher than that in glioma patients with low PTEN expression. C Pearson’s correlation analysis of the expression of circNALCN and PTEN in brain glioma. D, E The relative mRNA and protein levels of PTEN were detected by qRT-PCR and western blot, respectively, in U87 and U251 cells transfected with vector and miR-493-3pNC, oe-circNALCN and miR-493-3p mimics, oe-circNALCN and miR-493-3pNC, si-NC and miR-493-3p NC, si-circNALCN and miR-493-3p inhibitor, and si-circNALCN and miR-493-3p NC. ns indicates no significance. F The diagrammatic representation of our conclusion was shown. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Vector: empty vector control; NC: negative control oe-circNALCN: circNALCN overexpression vector; si-NC: negative control siRNA; si-circNALCN: circNALCN siRNA