Fig. 2

NEAT1 competitively combines to let-7 g. A The binding site of let-7 g and NEAT1 predicated using starBase and verified by a dual luciferase reporter gene assay. *p < 0.05 vs. the NC-mimic group. B The binding of NEAT1 and let-7 g with AGO2 in HO8910 cells tested by RIP assay. C The binding of NEAT1 and let-7 g with AGO2 in SKOV3 cells tested by RIP assay. * p < 0.05 vs. the IgG group. D The interaction of NEAT1 and let-7 g in HO8910 cells tested by RNA pull down assay. E The interaction of NEAT1 and let-7 g in SKOV3 cells tested by RNA pull down assay. *p < 0.05 vs. the Bio-probe NC group. F The let-7 g expression in HO8910 cells transfected with si-NEAT1 or oe-NEAT1 tested by RT-qPCR. G, The let-7 g expression in SKOV3 cells transfected with si-NEAT1 or oe-NEAT1 tested by RT-qPCR. *p < 0.05 vs. the oe-NC group, #p < 0.05 vs. the si-NC group. The measurement data were expressed as mean ± standard deviation. Comparisons between two groups were conducted by independent sample t-test, while comparisons between multiple groups were assessed by one-way ANOVA followed by Tukey's post hoc test. Cell experiments were repeated 3 times