Fig. 6

Silencing of NEAT1 restrains in vivo tumorigenesis by regulating the let-7 g/MEST/ATGL axis. Mice were treated with sh-NEAT1, let-7 g inhibitor or both. A The expression levels of NEAT1, let-7 g, MEST and ATGL in tumor tissues determined by RT-qPCR. B The protein expression of MEST and ATGL in tumor tissues determined by western blot analysis. C The positive expression of MEST and ATGL proteins in tumor tissues by immunohistochemistry (400 ×). D Representative images of tumors obtained after 40 d of tumor xenograft in nude mice. E Tumor growth curve. F The final tumor weight in each group. G Cell apoptosis of tumor tissues quantified by TUNEL staining (400 ×). *p < 0.05 vs. the si-NC + NC-inhibitor group. #p < 0.05 vs. the si-NEAT1 + NC-inhibitor group. Measurement data were depicted as mean ± standard deviation, and assessed by one-way ANOVA followed by Tukey's post hoc test. The data of each group at different time points were compared using repeated measures ANOVA followed by Bonferroni post hoc test