Fig. 1

Successful separation of CD133⁺ OCSCs. A A2780 cells were stained with APC-labeled CD133 antibody (x-axis), and CD133⁺ OCSCs were sorted using flow cytometry; B The morphological characteristics of CD133⁺ OCSCs cultured in serum-free medium for 5–6 days were observed under the microscope (100 ×); C, D Levels of CD133, CD44, NANOG and POU5F1 in A2780 cells and CD133⁺ OCSCs were detected by RT-qPCR and Western blot analysis. Data were analyzed using the unpaired t test. Compared with the A2780 cells, *p < 0.05, **p < 0.01. The experiment was performed in triplicate