Table 2 Primer sequence of RT-qPCR
Gene | Sequence 5′–3′ | |
|---|---|---|
CD133 | F: AGTCGGAAACTGGCAGATAGC | R: AGTCGGAAACTGGCAGATAGC |
LINC00115 | F: TGGCTTGTCTTCCATCGTCC | R: GCACGAGGGTTGTTACAGGA |
CD44 | F: CGGACACCATGGACAAGTTT | R: CCGTCCGAGAGATGCTGTAG |
GAPDH | F: CAAGATCATCACCAATGCCT | R: CCCATCACGCCACAGTTTCC |
NANOG | F: TTTGTGGGCCTGAAGAAAACT | R: AGGGCTGTCCTGAATAAGCAG |
POU5F1 | F: CTGGGTTGATCCTCGGACCT | R: CCATCGGAGTTGCTCTCCA |
miR-30a | F: GCGACTGTAAACATCCTCGAC | R: CAGCTGCAAACATCCGACTG |
SOX9 | F: AGGAAGTCGGTGAAGAACGG | R: AGGAAGTCGGTGAAGAACGG |
SOX9-3’UTR-Primer | F: CGGACTAGTACAGACCTTAATCTTAATTA | R: CCCAAGCTTTTTTTAATGCAATGTATATT |
SOX9-mut-Primer | F: GACTAGTTATTAACAGTTTTAGAAGTC | R: CAAGCTTAATGCAATGTATATTTATTG |
Luc-LINC00115wt-Primer | F: CGGACTAGTATCAGCAGAGATATGGAGGA | R: CCCAAGCTTGACAATCCAGGTCATCCTTC |
Luc-LINC00115mut-Primer | F: GACTAGTGTAGCAGAGAGGGCAGCAGA | R: CAAGCTTTTGATACTTATACTTAGTTG |
U6 | F: CTCGCTTCGGCAGCACATATACT | R: ACGCTTCACGAATTTGCGTGTC |