Fig. 2
MAZ promotes ccRCC cell proliferation. A SW839 and Caki cells were transfected with the pKLO, shMAZ, pWPI, and oeMAZ vectors, and then qRT-PCR was used to detect the level of MAZ mRNA. B SW839 and Caki cells were transfected as in A, and the MAZ protein level was detected by Western blot. C Quantitative analysis of B. D, E Cells were prepared as in A, and cell viability was measured by MTT (D) and colony formation assays (D–F). G SW839 cells engineered to stably overexpress shMAZ or negative control (pLKO) were injected subcutaneously in 200 µl PBS/Matrigel (50:50) into the mouse forelimb. At the final time point (21 days after injection), the tumor volumes in each group were measured after resection of tumors. H Tumor volume was determined by direct measurement with calipers and calculated by the formula: volume  =  [(length  ×  width2)/2]. All data are expressed as the mean  ±  SEM from three independent experiments. *P  <  0.05, **P  <  0.01, ***P  <  0.001 vs. the corresponding controls