Table 1 CaMK2 expression in numerous cancer progression is prominently changed reflected by bioinformatic analyses
From: The dysregulated expression and functional effect of CaMK2 in cancer
Cancer types | Method of bioinformatic analysis | The altered CaMK2 expression in cancer | References |
|---|---|---|---|
CRC | The integrated transcriptomic and proteomic datasets acquired from previous microarray- and mass spectrometry (MS)-based study | Both the transcription and protein levels of CaMK2δ are concordantly decreased during CRC progression | [27] |
The mRNA expression profile is inverstigated using GeneChip Human Transcriptome Array 2.0 | CaMK2β expression is downregulated in F. nucleatum-induced CRC | [28] | |
Total genome gene expression profiling is investigated using Illumina HT-12 V4.0 Expression Beadchip oligonucleotide microarray | CaMK2γ expression is significantly reduced in rectal cancer tissues compared with colon cancer tissues | [29] | |
GBM | The differentially expressed genes is found and validated by microarray analysis of RNA expression profiling and qRT-PCR, respectively | CaMK2β is significantly decreased in the MMLV/PDGFB mouse gliomas | [30] |
The gene expression in GBM and normal control are compared based on the data from GEPIA | The expression of CaMK2β and CaMK2γ are synchronously downregulated in GBM | [31] | |
Oligonucleotide-based microarray analysis and real-time RT-PCR verification | CaMK2γ transcript level in recurrent high-grade gliomas is significantly reduced as compared to primary low-grade gliomas | [32] | |
A prognostic prediction system was constructed and validated using microarray data from TCGA dataset, GEO dataset and a chinese Glioma Genome Atlas (CGGA) dataset | High CaMK2α mRNA expression may be a bad prognostic factor for GBM | [33] | |
HSCC | The differentially expressed genes that are closely related to the TPF chemosensitivity in priamry HSCC patients was screen using mRNA microarray analysis | The expressions of CaMK2α and CaMK2β were significantly increased in TPF-resistent patients compared with the TPF-sensitive patients | [34] |
MTNBC | The differentially expressed proteins between the chemosensitivity group and chemoresistance group were identified and validated by TMT-based proteomics analysis and ELISA analysis | The protein level of CaMK2α in chemoresistance group was significantly higher than that in chemosensitivity group | [35] |
NSCLC | A genome wide scan of single-nucleotide polymorphisms (SNPs) was conducted in patients with early-stage NSCLC | CaMK2δ rs10023113 was significantly associated with poor prognosis of early-stage NSCLC | [36] |
Breast cancer | The expression microarray data obtained from the EBI database was screened and then identified using a combinational approach involving in silico mining followed by MSP and RT-PCR | The promoter of CaMK2β was hypermethylazed and the expression of CaMK2β was decrased in breast cancer | [37] |
lingual SCC | The methylation of gene promoters in lingual SCC mouse model was determined using Methylated DNA immunoprecipitation sequencing | Significant methylation in CaMK2 promoter was confirmed in lingual SCC mouse model | [38] |
EAC | Targeted RNA sequencing and PCR verification were performed in 40 paired EAC specimens and nonmalignant specimens frompatients | The USP54- CaMK2γ fusion transcripts was found in EACA specimens | [39] |