Fig. 5

circDPP4 acts as a ceRNA for miR-195 in PCa cells. A Relative expression levels of circDPP4 in the nuclear and cytoplasmic fractions of PCa cells. B The predicted miR-195 binding sites in circDPP4. C Dual-luciferase reporter assay performed to validate the direct binding relationship between circDPP4 and miR-195. *P < 0.05 vs. mimics control-transfected cells. D The interaction between circDPP4 and miR-195 in PCa cells was measured by RNA-pull down assay. E RT-qPCR analysis of miR-195 expression levels in PC3 and LNCaP cells after transfection. *P < 0.05 vs. sh-NC or empty vector-transfected cells. F RT-qPCR analysis of miR-195 expression levels in 104 pairs of PCa tissues and adjacent normal tissues. *P < 0.05 vs. normal tissues. G RT-qPCR analysis of miR-195 expression levels in PCa cell lines and normal counterpart. *P < 0.05 vs. RWPE1 cells. H The inverse correlation between circDPP4 and miR-195 expression in PCa tissues of 20 randomly selected patients