Fig. 4

The overexpression of miR-146 promotes cell proliferation by targeting RARB. A, B The expression of RARB in cancer tissues and the corresponding adjacent tissues was determined by immunohistochemical staining and qRT-PCR, respectively. *P < 0.05 vs. adjacent tissues. adj, adjacent tissues; can, cancer tissues. C The relationship between RARB and HAND2-AS1 expression in 32 pairs of bladder cancer tissues. D Left panel, schematic of the predicted binding sites of miR-146 and RARB; Right panel, the relative activity of the RARB promoter was determined by dual-luciferase assay. RARB, RARB wild type 3′-UTR; RARB Mut, RARB mutated 3′-UTR; miR-146 in, miR-146 inhibitor. **P < 0.01, vs. scramble RNA, n = 3. E The expression of RARB or miR-146 in the indicated cells was analyzed by qRT-PCR. *P < 0.05, vs. scrambled RNA, n = 3. Scr, scrambled RNA. F The expression of RARB in the indicated cells was analyzed by western blot. G The proliferation of 5637 and RT4 cells expressing miR-146in, sh-RARB or the combination was accessed by MTT assay. miR-146 in, miR-146 inhibitor. sh-RARB, shRNA targeting RARB. *P < 0.05, vs. control, n = 3. Scr, scramble RNA