Fig. 1
From: β-Catenin/LEF-1 transcription complex is responsible for the transcriptional activation of LINC01278
LEF-1 binds to the promoter region of the LINC01278 gene. A The putative LEF-1 binding site on LINC01278 promoter sequence determined by online PROMO algorithm. B The luciferase activity of TPC-1 and BCPAP cells which were co-transfected with LINC01278-WT and LEF-1, or LEF-1 and LINC01278-MUT detected by luciferase reporter assays. C The immunoprecipitated promoter fragment containing the LEF-1 response element was probed by PCR using primers targeting the regulatory region of LINC01278 gene. D and E The protein expression of LEF-1 in TPC-1 and BCPAP cells which were transfected with LEF-1 siRNA (siRNA) or overexpression plasmid (LEF-1). F The relative expression of LINC01278 in TPC-1 and BCPAP cells which were transfected with LEF-1 siRNA (siRNA) or overexpression plasmid (LEF-1). *P < 0.05